Kinetic data for SABIO kinetic law id 14792

General information
Organism Homo sapiens
Tissue -
EC Class 2.7.1.6
SABIO reaction id 1354
Variant wildtype His-tagged
Recombinant expressed in Escherichia coli HMS174(DE3)
Experiment Type in vitro
Pathways Amino sugar and nucleotide sugar metabolism
Biosynthesis of nucleotide sugars
Galactose metabolism
Metabolic pathways
Event Description -
Substrates
name location comment
D-Galactose cytosol -
ATP cytosol -
Products
name location comment
alpha-D-Galactose 1-phosphate cytosol -
ADP cytosol -
Modifiers
name location effect comment protein complex
galactokinase(Enzyme) cytosol Modifier-Catalyst - P51570;
Enzyme (protein data)
UniProtKB_AC name mol. weight (kDa) deviation (kDa)
subunit P51570 - - -
complex - - - -
Kinetic Law
type formula annotation
Michaelis-Menten (E*kcat*A)/(Km+A) SBO:0000029
Parameter
name type species start val. end val. deviat. unit comment
E concentration Enzyme 32.0 67.0 - nM -
A concentration ATP 0.0 80.0 - µM -
kcat kcat - 8.7 - 5 s^(-1) -
kcat_Km kcat/Km ATP 260000.0 - 0.4E5 M^(-1)*s^(-1) -
Km Km ATP 34.0 - 4 µM -
Experimental conditions
start value end value unit
temperature 37.0 - °C
pH 8.0 - -
buffer 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
comment -
Reference
title author year journal volume pages PubMed
Functional analysis of disease-causing mutations in human galactokinase Timson DJ, Reece RJ 2003 Eur J Biochem 270 1767-74 12694189
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