Enzyme Details

EC Classification

3.5.1.11

Enzyme name
(recommended)

penicillin amidase

Alternative names

penicillin amidohydrolase
penicillin acylase
benzylpenicillin acylase
novozym 217
semacylase
alpha-acylamino-beta-lactam acylhydrolase
ampicillin acylase

Classification

Hydrolases

Acting on carbon-nitrogen bonds, other than peptide bonds

In linear amides

Catalyses reactions

H2O + Penicillin <-> Carboxylate + 6-Aminopenicillanate

H2O + 2-Phenylacetamide <-> NH3 + Phenylacetic acid

H2O + Penicillin G <-> 2-Phenyl acetate + 6-Aminopenicillanate

H2O + 2-Nitro-5-[(Phenylacetyl)amino]benzoic acid <-> Phenylacetic acid + 5-Amino-2-nitrobenzoic acid

H2O + D-Phenylglycine amide <-> NH3 + D-Phenylglycine

H2O + Ampicillin <-> 6-Aminopenicillanate + D-Phenylglycine

H2O + D-Phenylglycine methyl ester <-> Methanol + D-Phenylglycine

H2O + Cefadroxil <-> D-4-Hydroxyphenylglycine + 7-Desacetoxycephalosporanic acid

H2O + Amoxicillin <-> 6-Aminopenicillanate + D-4-Hydroxyphenylglycine

H2O + Cephalexin <-> 7-Desacetoxycephalosporanic acid + D-Phenylglycine

H2O + D-4-Hydroxyphenylglycine amide <-> NH3 + D-4-Hydroxyphenylglycine

H2O + D-4-Hydroxyphenylglycine methyl ester <-> Methanol + D-4-Hydroxyphenylglycine

H2O + Methyl 2-phenylacetate <-> Methanol + Phenylacetic acid

H2O + p-Nitrophenyl phenylacetate <-> 2-Phenyl acetate + p-Nitrophenol

H2O + D-(-)-alpha-Aminophenylacetic acid 4-nitroanilide <-> 4-Nitroaniline + 2-Amino-2-phenylacetate

H2O + Benzylpenicilloic acid <-> 2-Phenyl acetate + 6-Aminopenicilloic acid

H2O + Ampicillin <-> 6-Aminopenicillanate + 2-Amino-2-phenylacetate

H2O + Phenylacetylglycine <-> Glycine + 2-Phenyl acetate

H2O + Cephalexin <-> 3-Methyl-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + 2-Amino-2-phenylacetate

H2O + Cephaloridine <-> Thien-2-ylacetate + 7-Amino-8-oxo-3-(pyridin-1-ium-1-ylmethyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate

H2O + Cephalothin <-> Thien-2-ylacetate + 7-Aminocephalosporanic acid

H2O + Phenylacetate 4-nitroanilide <-> 2-Phenyl acetate + 4-Nitroaniline

H2O + Phenylacetyl-7-aminodeacetoxy cephalosporanic acid <-> 2-Phenyl acetate + 7-Aminodeacetoxy cephalosporanic acid

H2O + Ethyl 2-phenylacetate <-> Ethanol + 2-Phenyl acetate

External links

Expasy
KEGG
BRENDA
IntEnz (EBI)
IUBMB
Reactome
MetaCrop

View all entries for EC Class: 3.5.1.11