Enzyme Details

Enzyme (Recommended) Name	cholesterol 24-hydroxylase
Alternative names	cholesterol,NADPH:oxygen oxidoreductase (24-hydroxylating) cholesterol 24-monooxygenase CYP46 CYP46A1 cholesterol 24S-hydroxylase cytochrome P450 46A1
EC Classification	1.14.14.25
Classification	Oxidoreductases Acting on paired donors with incorporation of molecular oxygen With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
Catalyses reactions	H+ + NADPH + O2 + Cholesterol <-> H2O + NADP+ + 24(S)-Hydroxycholesterol H+ + NADPH + O2 + 24(S)-Hydroxycholesterol <-> H2O + NADP+ + Dihydroxycholesterol H+ + NADPH + O2 + Bufuralol <-> H2O + NADP+ + 1-Hydroxy bufuralol H+ + NADPH + O2 + Progesterone <-> H2O + NADP+ + 17alpha-Hydroxyprogesterone H+ + NADPH + O2 + Dextromethorphan <-> H2O + NADP+ + 3-Methoxydextrorphanin H+ + NADPH + O2 + Dextromethorphan <-> H2O + NADP+ + Dextrorphan H+ + NADPH + O2 + Cortisol <-> Unknown + H2O + NADP+ H+ + NADPH + O2 + Diclofenac <-> H2O + NADP+ + 4'-Hydroxydiclofenac H+ + NADPH + O2 + Phenacetin <-> H2O + Ethanol + NADP+ + Acetaminophen H+ + NADPH + O2 + Testosterone <-> H2O + NADP+ + 6beta-Hydroxytestosterone H+ + NADPH + O2 + Testosterone <-> H2O + NADP+ + 2-Hydroxytestosterone H+ + NADPH + O2 + Testosterone <-> H2O + NADP+ + 16beta-Hydroxytestosterone
External links	Expasy KEGG IntEnz (EBI) IUBMB Reactome HepatoSys
	View all entries for EC Class 1.14.14.25