Enzyme Details

Enzyme (Recommended) Name	cholesterol monooxygenase (side-chain-cleaving)
Alternative names	cholesterol,reduced-adrenal-ferredoxin:oxygen oxidoreductase (side-chain-cleaving) cholesterol desmolase cytochrome P-450scc desmolase, steroid 20-22 C27-side chain cleavage enzyme cholesterol 20-22-desmolase cholesterol C20-22 desmolase cholesterol side-chain cleavage enzyme cholesterol side-chain-cleaving enzyme enzymes, cholesterol side-chain-cleaving steroid 20-22 desmolase steroid 20-22-lyase
EC Classification	1.14.15.6
Classification	Oxidoreductases Acting on paired donors with incorporation of molecular oxygen With a reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen
Catalyses reactions	H+ + NADPH + O2 + Cholesterol <-> H2O + NADP+ + 20alpha-Hydroxycholesterol H+ + NADPH + O2 + Cholesterol <-> H2O + NADP+ + 22beta-Hydroxycholesterol O2 + Cholesterol + Reduced adrenal ferredoxin <-> H2O + Oxidized adrenal ferredoxin + 4-Methylpentanal + Pregnenolone O2 + Ferredoxin + 20alpha,22beta-Dihydroxycholesterol <-> Ferredoxin + 4-Methylpentanal + Pregnenolone H+ + NADPH + O2 + 17alpha,20alpha-Dihydroxycholesterol <-> H2O + NADP+ + 4-Methylpentanal + 17alpha-Hydroxypregnenolone H+ + NADPH + O2 + 20alpha-Hydroxycholesterol <-> H2O + NADP+ + 20alpha,22beta-Dihydroxycholesterol H+ + NADPH + O2 + 22beta-Hydroxycholesterol <-> H2O + NADP+ + 20alpha,22beta-Dihydroxycholesterol O2 + Reduced adrenal ferredoxin + Cholesta-5,7-dien-3beta-ol <-> H2O + Oxidized adrenal ferredoxin + 4-Methylpentanal + 7-Dehydropregnenolone O2 + Reduced ferredoxin + Cholest-5-ene-3beta,25-diol <-> H2O + Oxidized ferredoxin + Pregnenolone + 4-Methyl-4-hydroxypentanal
External links	Expasy KEGG IntEnz (EBI) IUBMB Reactome HepatoSys
	View all entries for EC Class 1.14.15.6