Kinetic data for SABIO kinetic law id 14111

General information
Organism Homo sapiens
Tissue prostate cancer cell
EC Class 1.1.1.22
SABIO reaction id 784
Variant mutant C276S
Recombinant expressed in Escherichia coli Rosetta(DE3)pLysS
Experiment Type in vitro
Pathways Amino sugar and nucleotide sugar metabolism
Ascorbate and aldarate metabolism
Biosynthesis of cofactors
Biosynthesis of nucleotide sugars
Metabolic pathways
O-Antigen nucleotide sugar biosynthesis
Pentose and Glucuronate interconversions
Starch and Sucrose metabolism
Event Description -
Substrates
name location comment
UDPglucose - -
H2O - -
NAD+ - -
Products
name location comment
NADH - -
UDPglucuronate - -
H+ - -
Modifiers
name location effect comment protein complex
UDP-glucose 6-dehydrogenase(Enzyme) - Modifier-Catalyst - (O60701)*6;
Enzyme (protein data)
UniProtKB_AC name mol. weight (kDa) deviation (kDa)
subunit O60701 - 57.0 -
complex - - 345.0 -
Kinetic Law
type formula annotation
- - -
Parameter
name type species start val. end val. deviat. unit comment
A concentration NAD+ 0.0 1.5 - mM -
Kd Kd NAD+ 127.0 - 4.0 µM -
Experimental conditions
start value end value unit
temperature 22.0 - °C
pH 7.4 - -
buffer 0.1 M Tris-HCl
comment -
Reference
title author year journal volume pages PubMed
Characterization of human UDP-glucose dehydrogenase. CYS-276 is required for the second of two successive oxidations Sommer BJ, Barycki JJ, Simpson MA 2004 J Biol Chem 279 23590-6 15044486
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