Kinetic data for SABIO kinetic law id 26290

General information
Organism Homo sapiens
Tissue -
EC Class 2.7.1.6
SABIO reaction id 1354
Variant wildtype His-tagged
Recombinant expressed in Escherichia coli
Experiment Type in vitro
Pathways Amino sugar and nucleotide sugar metabolism
Biosynthesis of nucleotide sugars
Galactose metabolism
Metabolic pathways
Event Description -
Substrates
name location comment
ATP - -
D-Galactose - -
Products
name location comment
ADP - -
alpha-D-Galactose 1-phosphate - -
Modifiers
name location effect comment protein complex
galactokinase(Enzyme) - Modifier-Catalyst - P51570;
Enzyme (protein data)
UniProtKB_AC name mol. weight (kDa) deviation (kDa)
subunit P51570 - - -
complex - - - -
Kinetic Law
type formula annotation
Michaelis-Menten kcat*E*S/(Km+S) SBO:0000029
Parameter
name type species start val. end val. deviat. unit comment
E concentration Enzyme - - - - -
S concentration D-Galactose 0.0 2000.0 - µM -
kcat kcat - 8.7 - 0.5 s^(-1) -
kcat_Km kcat/Km D-Galactose 8900.0 - 2900 M^(-1)*s^(-1) -
Km Km D-Galactose 970.0 - 220 µM -
Experimental conditions
start value end value unit
temperature 37.0 - °C
pH 8.0 - -
buffer 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
comment -
Reference
title author year journal volume pages PubMed
Sugar recognition by human galactokinase Timson DJ, Reece RJ 2003 BMC Biochem 4 1-8 14596685
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