|
General information |
| Organism |
Rattus norvegicus
|
| Strain |
Lewis |
| Tissue |
liver
|
| EC Class |
3.5.1.12 |
| SABIO reaction id |
12508 |
| Variant |
wildtype thiol-type
|
| Experiment Type |
in vitro |
| Event Description |
- |
|
|
|
|
|
| Modifiers |
| name |
location |
effect |
comment |
protein complex |
| biotinidase(Enzyme)
|
membrane
|
Modifier-Catalyst |
- |
Q5FVF9;
|
|
| Enzyme (protein data) |
|
UniProtKB_AC |
name |
mol. weight (kDa) |
deviation (kDa) |
| subunit |
- |
- |
- |
- |
| complex |
- |
- |
66.0 |
- |
|
| Kinetic Law |
type
|
formula
|
annotation |
| Michaelis-Menten |
Vmax*S/(Km+S)
|
SBO:0000029
|
|
| Parameter |
| name |
type |
species |
start val. |
end val. |
deviat. |
unit |
comment |
| S |
concentration
|
Biotinyl-6-aminoquinoline |
5.0 |
20.0 |
- |
µM |
- |
| kcat_Km |
kcat/Km
|
Biotinyl-6-aminoquinoline |
8.28 |
- |
- |
M^(-1)*s^(-1) |
- |
| Km |
Km
|
Biotinyl-6-aminoquinoline |
15.4 |
- |
- |
µM |
- |
| Vmax |
Vmax
|
- |
101.0 |
- |
- |
pmol/(min*mg) |
- |
|
| Experimental conditions |
|
start value |
end value |
unit |
| temperature |
37.0 |
- |
°C
|
| pH |
7.0 |
- |
-
|
| buffer |
0.1 M Tris-HCl, 10 mM 2-mercaptoethanol, 1 mM EDTA, 10% (v/v) glycerol |
|
|
|
| Reference |
| title |
author |
year |
journal |
volume |
pages |
PubMed |
| Determination of specific activities and kinetic constants of biotinidase and lipoamidase in LEW rat and Lactobacillus casei (Shirota) |
Hayakawa K, Guo L, Terentyeva EA, Li XK, Kimura H, Hirano M, Yoshikawa K, Nagamine T, Katsumata N, Ogata T, Tanaka T |
2006 |
J Chromatogr B Analyt Technol Biomed Life Sci |
844 |
240-50 |
16876490
|
report wrong or missing data
|