Kinetic data for SABIO kinetic law id 45443

General information
Organism Anas platyrhynchos
Tissue -
EC Class 4.3.2.1
SABIO reaction id 573
Variant mutant delta-2 crystallin-W9A
Experiment Type in vitro
Pathways Alanine, aspartate and glutamate metabolism
Arginine and Proline metabolism
Arginine biosynthesis
Biosynthesis of amino acids
Biosynthesis of secondary metabolites
Insulin signaling pathway
Metabolic pathways
Urea cycle
Event Description -
Substrates
name location comment
N(omega)-(L-Arginino)succinate - Disodium argininosuccinate
Products
name location comment
Fumarate - -
L-Arginine - -
Modifiers
name location effect comment protein complex
argininosuccinate lyase(Enzyme) - Modifier-Catalyst purified (P24058)*4;
Enzyme (protein data)
UniProtKB_AC name mol. weight (kDa) deviation (kDa)
subunit - - - -
complex - - - -
Kinetic Law
type formula annotation
Michaelis-Menten Vmax*A/(Km+A) SBO:0000029
Parameter
name type species start val. end val. deviat. unit comment
E concentration Enzyme 0.0125 - - mg/ml -
A concentration N(omega)-(L-Arginino)succinate 0.02 2.0 - mM -
kcat kcat - 0.155 - 0.090 s^(-1) -
kcat_Km kcat/Km N(omega)-(L-Arginino)succinate 1360.0 - 0.020E4 M^(-1)*s^(-1) -
Km Km N(omega)-(L-Arginino)succinate 0.112 - 0.025 mM -
Vmax Vmax - 0.04 - 0.001 µmol/(min*mg) -
Experimental conditions
start value end value unit
temperature 25.0 - °C
pH 7.5 - -
buffer 10 mM Tris-HCl, 1 mM EDTA
Reference
title author year journal volume pages PubMed
Recovery of argininosuccinate lyase activity in duck delta1 crystallin Tsai M, Koo J, Howell PL 2005 Biochemistry 44 9034-44 15966727
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