Kinetic data for SABIO kinetic law id 5046

General information
Organism Homo sapiens
Tissue -
EC Class 2.1.1.45
SABIO reaction id 88
Variant wildtype
Recombinant expressed in Escherichia coli
Experiment Type in vitro
Pathways Folate biosynthesis
Metabolic pathways
One carbon pool by folate
Pyrimidine metabolism
Event Description -
Substrates
name location comment
dUMP - -
5,10-Methylenetetrahydrofolate - -
Products
name location comment
dTMP - -
Dihydrofolate - -
Modifiers
name location effect comment protein complex
E-5-(2-Bromovinyl)-2'-deoxyuridine monophosphate - Modifier-Inhibitor active species of anticancer drug NB1011 -
thymidylate synthase(Enzyme) - Modifier-Catalyst - (P04818)*2;
Enzyme (protein data)
UniProtKB_AC name mol. weight (kDa) deviation (kDa)
subunit P04818 - - -
complex - - - -
Kinetic Law
type formula annotation
Competitive inhibition E*kcat*A/(Km_A*(1+I/Ki)+A) SBO:0000260
Parameter
name type species start val. end val. deviat. unit comment
A concentration dUMP 2.0 32.0 - 然 -
I concentration E-5-(2-Bromovinyl)-2'-deoxyuridine monophosphate 0.0 40.0 - 然 -
E concentration Enzyme 0.1 - - 然 -
B concentration 5,10-Methylenetetrahydrofolate 50.0 - - 然 saturated
kcat kcat - 0.213 - 0.020 s^(-1) -
kcatKm_A kcat/Km dUMP 79000.0 - - M^(-1)*s^(-1) -
Ki Ki E-5-(2-Bromovinyl)-2'-deoxyuridine monophosphate 4.17 - 0.29 然 -
Km_A Km dUMP 2.7 - 0.33 然 -
Experimental conditions
start value end value unit
temperature 30.0 - °C
pH 7.5 - -
buffer 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA, 100 mM beta-mercaptoethanol
comment -
Reference
title author year journal volume pages PubMed
Kinetic properties of human thymidylate synthase, an anticancer drug target Sergeeva OA, Khambatta HG, Cathers BE, Sergeeva MV 2003 Biochem Biophys Res Commun 307 297-300 12859954
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